Valorization of waste foods using pullulan hydrolase from Thermococcus kodakarensis
نویسندگان
چکیده
منابع مشابه
Novel maltotriose-hydrolyzing thermoacidophilic type III pullulan hydrolase from Thermococcus kodakarensis.
A novel thermoacidophilic pullulan-hydrolyzing enzyme (PUL) from hyperthermophilic archaeon Thermococcus kodakarensis (TK-PUL) that efficiently hydrolyzes starch under industrial conditions in the absence of any additional metal ions was cloned and characterized. TK-PUL possessed both pullulanase and α-amylase activities. The highest activities were observed at 95 to 100°C. Although the enzyme ...
متن کاملThermococcus kodakarensis DNA replication.
DNA replication plays an essential role in all life forms. Research on archaeal DNA replication began approximately 20 years ago. Progress was hindered, however, by the lack of genetic tools to supplement the biochemical and structural studies. This has changed, however, and genetic approaches are now available for several archaeal species. One of these organisms is the thermophilic euryarchaeo...
متن کاملGenetics Techniques for Thermococcus kodakarensis
Thermococcus kodakarensis (T. kodakarensis) has emerged as a premier model system for studies of archaeal biochemistry, genetics, and hyperthermophily. This prominence is derived largely from the natural competence of T. kodakarensis and the comprehensive, rapid, and facile techniques available for manipulation of the T. kodakarensis genome. These genetic capacities are complemented by robust p...
متن کاملAn archaeal histone is required for transformation of Thermococcus kodakarensis.
Archaeal histones wrap DNA into complexes, designated archaeal nucleosomes, that resemble the tetrasome core of a eukaryotic nucleosome. Therefore, all DNA interactions in vivo in Thermococcus kodakarensis, the most genetically versatile model species for archaeal research, must occur in the context of a histone-bound genome. Here we report the construction and properties of T. kodakarensis str...
متن کاملMolecular cloning and characterization of TK1111, a cupin-type phosphoglucose isomerase from Thermococcus kodakarensis
Aim: To characterize, TK1111, a cupin-type phosphoglucose isomerase from a hyperthermophilic archaeon Thermococcus kodakarensis. Methods: The genome of T. kodakarensis was searched in order to find an open reading frame annotated as glucose-6-phosphate isomerase. The structural gene was cloned, expressed in Escherichia coli and the purified gene product was characterized. Effect of metal ions o...
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ژورنال
عنوان ژورنال: Amylase
سال: 2018
ISSN: 2450-9728
DOI: 10.1515/amylase-2018-0005